FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926965
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13450.013.5473359
CGTTGGA21950.013.0392531
CGGGATT12600.012.14030210
AAACGGG22950.012.0562877
AACGGGA18550.011.7630238
GAAACGG22700.011.695056
GGAAACG24000.010.9850815
TAATCCG1903.819468E-410.6737635
CGGGATA3053.7737973E-69.58814610
CGCGCCC5007.4578566E-119.44801133
AACGGGT5901.8189894E-129.1506188
CGAAACG2000.00696508879.01345
CCCGCGC2050.0085191198.7946721
ACCGCGC6700.08.729506531
CCCGTAT2902.158552E-48.548271
CGTGAGC21850.08.54511423
CACCGCG6851.8189894E-128.53834930
GCCACCG16750.08.46090628
CCGCGCC7250.08.37754732
GCGTGAG20750.08.34764122
Produced by FastQC (version 0.11.5)