FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7342602
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5350.023.12549642
CGTATGC5500.022.903844
TCTCGTA5350.022.28456941
GATCGGA6450.019.918461
ATCGGAA7100.018.4123952
TCGTATG7000.017.35313243
TCGGAAG7850.016.9385243
ACACGTC8150.015.7325613
ATCTCGT7700.015.48343540
CGTCTGA8400.014.19314916
TCACGAT8250.014.17873435
CGGAAGA9850.013.4992294
CGTTGGA25250.013.0326321
CACGTCT10400.012.54517714
CGCGCCC4550.011.8655533
GTATGCC11600.011.836658545
AAACGGG26800.011.8349517
ACGATCT10300.011.79355137
AACGGGA22350.011.6751538
CACGATC10250.011.63161736
Produced by FastQC (version 0.11.5)