FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8967638
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7850.022.35334642
TCTCGTA8800.020.19584341
CGTATGC8900.019.4634944
ATCTCGT9750.018.2279440
TCGTATG9900.017.95196243
GATCGGA10050.017.715631
ATCGGAA10650.016.910292
TCGGAAG11450.015.5313953
ACACGTC11500.015.45524413
CGTTGGA24550.014.6880471
CGGGATT16150.013.79127510
CGTCTGA13050.013.79111816
ACGGGAT16800.013.52551659
AATCTCG13250.013.24322939
AACGGGA22200.013.0722968
AAACGGG24900.012.830057
CGGAAGA14850.012.7333214
ACGTCTG14150.012.71979615
GTATGCC14450.012.61085245
GAAACGG26750.012.1174336
Produced by FastQC (version 0.11.5)