FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11336234
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3750.012.59997645
ACGCCCG4900.012.39722534
TCTCGTA5150.012.232285541
CCGGCTA10650.011.61903138
CTCGTAT5500.011.04543442
CGTTGGA30300.010.9241091
AAACGGG31250.010.8717887
CGGCTAA11050.010.58760839
GATCGGA5550.010.5485241
AACGGGA25800.010.2901798
ACGGGAT19700.010.278679
GAAACGG33500.010.0074886
CGTATGC6450.09.76742444
CGCCCGG10150.09.75311835
CCCGTAA4551.2714736E-99.4027211
GGAAACG35050.09.3081555
CGGGATT19050.08.85778210
TCGTATG7700.08.76621743
AACGGGT7250.08.6892178
ACAGGCG34450.08.2941418
Produced by FastQC (version 0.11.5)