FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8527844
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.021.74076845
TCTCGTA4900.019.2864740
CGTATGC5350.018.50453243
CTCGTAT5300.017.83005141
TCGTATG6550.014.7708842
ATCTCGT7250.013.03499339
AAACGGG25500.012.616817
CGTTGGA25900.012.2538261
ATCGGAA7600.012.1429061
GAAACGG28250.011.5477716
AACGGGA21550.011.4841588
ACACGTC8350.011.04820812
GGAAACG28750.010.9583025
TCGGAAG8500.010.8571862
ACGGGAT16200.010.8327429
AACGGGT6700.010.7455568
CGGGATT15300.010.58765710
CGTCTGA9150.010.32809215
ACAGGCG28950.010.18144818
CCCGAAA3104.2032116E-710.1652651
Produced by FastQC (version 0.11.5)