FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11795826
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16100.032.70038645
CTCGTAT16350.031.65262843
TCTCGTA16950.030.39930242
TATCTCG17150.029.65081440
GTCACGA18000.028.37299328
TCGTATG18700.027.6758344
ATCTCGT18600.027.46087841
AGTCACG19350.026.85856627
ATCGGAA19700.026.2850071
TCGGAAG20500.025.4712262
ACACGTC20500.025.24126212
CACGAGT20450.025.19381930
ACGAGTG20700.024.99876431
CGTCTGA21350.024.76374215
ACGTCTG22950.023.13532614
CGGAAGA23250.022.7469833
CACGTCT24500.021.4876413
GCACACG26000.020.33613610
CACACGT27850.019.22762711
TCACGAG27400.019.0497529
Produced by FastQC (version 0.11.5)