FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5961478
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA16500.012.8161578
AAACGGG20300.012.5226537
ACGGGAT11150.012.5092359
GTCACGA3457.2759576E-1212.39069928
CGGGATT10600.012.0974510
TATCTCG3203.783498E-1011.95163940
CGTTGGA18950.011.7972881
GAAACGG21200.011.4607426
TCTCGTA3703.601599E-1010.94559542
CTCGTAT3602.6630005E-910.62448143
CGAAACG1700.001841423110.586835
ATCTCGT3909.0039975E-1010.38384741
CGGGAAT7650.010.29275110
ACGGGTA2201.3135471E-410.2256579
GGAAACG22300.010.08834655
CGTATGC4601.5679689E-99.29372645
CCCGACA2453.7347095E-49.21701051
AGTCACG4752.7994247E-98.99978627
ACCACGC12050.08.96139131
CGCGATC2050.0086401448.779469521
Produced by FastQC (version 0.11.5)