FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058260
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8750.033.15870342
TCTCGTA9100.031.88336441
TATCTCG9150.031.70861439
CGATGTA9450.031.65399434
ACCGATG9650.031.2310232
CCGATGT9850.030.59688833
CACCGAT10250.030.06113431
CGTATGC9750.029.75805344
ATCTCGT9950.029.1591840
GTCACCG10700.028.37625529
GATCGGA10700.028.256781
TCGTATG10500.027.63247943
TCGGAAG11700.026.4202163
TCACCGA11700.026.14316230
ACACGTC12200.025.62477713
ATCGGAA11950.025.4898622
CGTCTGA12300.025.23359316
ACGTCTG13850.022.73439415
CACGTCT14050.022.57084814
CGGAAGA13700.022.2338584
Produced by FastQC (version 0.11.5)