FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9433675
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC7600.012.43292531
AAACGGG26450.011.9080957
AACGGGA21200.011.8855648
CGTTGGA28200.011.7502441
CGCGCCC6150.011.70623133
CCGCGCC8450.010.91614832
GAAACGG29100.010.7494466
GGAAACG29700.010.456685
ACGGGAT17050.010.4240889
CACCGCG8450.010.38458830
ACGCCCG5101.8189894E-1210.14611734
CGGGATT16050.09.95224310
GCCACCG20600.09.72069528
CGCCCGG10050.09.62607635
CGTAGAT3309.691139E-79.5629691
GCGTGAG23650.09.5127622
CGGGAAT8700.09.05078910
CGTGAGC24900.09.03521123
CCCGATA2250.0017950489.0165151
CCCCGAT3806.845348E-78.8978761
Produced by FastQC (version 0.11.5)