FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6527260
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18350.012.0472041
AACGGGA16750.011.4160998
GGAAACG20700.011.08919055
ACGGGAT12450.011.0225169
GAAACGG21850.010.8146186
AAACGGG20450.010.5607527
CGGGATT11500.09.97683110
CCCCGTA2057.6136965E-49.9034131
CCCGGAT2352.484931E-49.5990521
GCCACCG12750.09.35188728
ACCGCGC3807.0385795E-78.88057831
TCTCGTA2801.5035701E-48.83815441
CGTTAAA4102.1422602E-78.8030341
CGCGCCC3155.7248424E-58.57013433
ACAGGCG22850.08.46867918
GCGTGAG15200.08.28993622
CCACCGC9800.08.26437529
CCGCGCC3858.000452E-68.18089632
CGGGAAT6653.6379788E-118.11913710
CCCAAAG72450.08.0329961
Produced by FastQC (version 0.11.5)