FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5250118
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC2302.5465852E-1115.64653733
CCGCGCC3550.013.9386432
ACCGCGC3450.013.69058831
CACCGCG3950.013.09642230
GCCACCG10800.012.91201428
GCGTGAG12100.012.82595622
CGTGAGC12650.012.8017123
CGCCCGG4250.012.70130435
CGTTGGA13900.012.4953591
AAACGGG14100.011.6446297
TAGGCGT1751.8128719E-411.56726119
GAAACGG15000.011.5466096
AACGGGA12600.011.4243468
ACTGCGC3002.2439053E-811.24584131
GGAAACG15350.011.1649745
GCGCCCG3451.3242243E-911.08296334
GCCCGGC9750.011.07293336
ACAGGCG15500.011.02828418
ATAGGCG2251.3672405E-510.996037518
AGGCGTG15150.010.98614120
Produced by FastQC (version 0.11.5)