FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297038
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC2853.6379788E-1214.21089333
CGGGATA2257.40838E-812.99886210
ACCGCGC3753.8198777E-1111.40072531
ACGGGAT9900.011.3615689
TATGCCG2004.981178E-511.25060845
AACGGGA12700.011.1593888
CGCCCGG5300.011.03802135
CGGCCTA2503.7010705E-610.79905539
CGTGAGC13550.010.45945123
GCGTGAG13800.010.43288522
CGTTGGA15150.010.2718411
CCGCGCC3951.1204975E-910.25352632
CACCGCG3951.1204975E-910.25352630
CTCGTAT2201.3115471E-410.22715141
AAACGGG15350.010.1133137
CGGGATT8850.09.660171510
TCTCGTA2352.5499158E-49.57435340
GAAACGG16500.09.5464236
ACAGGCG19100.09.42210418
GCGCCCG4152.7292117E-89.21710534
Produced by FastQC (version 0.11.5)