FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12481068
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1552891.2441964101149037TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA171850.043.2683371
CTCGTAT177050.041.5492242
TCTCGTA177600.041.43321641
CGTATGC178550.041.3387644
ATCGGAA180450.041.2467422
TCGTATG180500.040.95447543
ATCTCGT180150.040.88419740
TCGGAAG182600.040.833143
ACACGTC182250.040.75856413
CATCTCG181300.040.5254439
CGTCTGA183100.040.47105816
CGGAAGA186550.040.330644
ACGTCTG186150.039.80795715
GTATGCC187050.039.49171445
CACGTCT188000.039.42819214
GCACACG191250.039.19339811
CACACGT193150.038.79620412
GATCATC198200.037.1266936
AGTCACC204700.036.16771728
ACCAGAT205550.036.01815432
Produced by FastQC (version 0.11.5)