FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7530444
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA800.001692743316.9036061
CGTTGGA18100.014.1952941
AACGGGA16450.012.7201848
AAACGGG20100.012.5365537
ACGGGAT11750.012.2551479
GAAACGG21500.011.7206866
GGAAACG21500.011.7206865
CCCCGAA3306.1299943E-1011.61055851
CGGGATT12250.011.38759310
CGCGCCC4702.1827873E-1110.05264933
CCCCGAT2751.1292323E-59.83482551
ACCGCGC6350.09.56641331
CCCGAAA3155.5527344E-69.3014551
CCGGCTA8000.09.28068738
CGGCTAA8150.09.10987739
CGGGAAT8650.09.10391710
CACCGCG6500.08.99951530
AACGGGT4551.2811142E-88.9009728
ACCCGGC7050.08.61649836
CGTAAGA2902.1566552E-48.548951
Produced by FastQC (version 0.11.5)