FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8598120
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA20150.014.6229488
CGTTGGA24450.014.2997451
AAACGGG26800.013.9319537
ACGGGAT15300.013.2309139
GAAACGG27550.012.6572616
ACGCCCG4800.012.6520634
CGGGATT14650.012.28262110
CGCCCGG8850.011.94519635
GGAAACG29750.011.5919325
CGGGAAT10200.010.14369910
ACCGCGC5301.8189894E-129.76091931
GCGTGAG19450.09.48470722
ACGGGAA10350.09.1274129
CGGGATA3603.251098E-68.74710410
AACGGGT7550.08.6395238
CGATAGG2350.0026476638.6387341
GCCCGGC18550.08.6090136
CGTGAGC20700.08.58591123
CCCAAAG93000.08.5133321
GCGCCCG6351.2732926E-118.501121534
Produced by FastQC (version 0.11.5)