FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11360390
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4900.016.98939745
CGTTGGA31500.013.6510091
TCTCGTA6700.013.43287540
AAACGGG32700.012.9354767
ACGGGAT19850.012.808499
CTCGTAT6750.012.66703841
GAAACGG33500.012.626576
AACGGGA27950.012.3165658
CGGGATT18800.011.60892310
GGAAACG35700.011.599385
CGTATGC7850.011.46490143
ACGCCCG5800.011.24978534
CGACGAT1450.006800605410.868561
ATCTCGT7950.010.75474839
ACCGCGC7750.010.74163631
CACCGCG8650.010.40405830
CGCCCGG11300.010.15471835
TCGTATG8250.010.0908542
TCGGAAG8950.010.0582862
CCGCGCC8600.09.67996332
Produced by FastQC (version 0.11.5)