FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6011297
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA15800.014.09176358
AAACGGG17650.013.8889387
ACGGGAT11700.013.839939
CGTTGGA18650.013.6841731
CGGGATT10800.013.119110
ACGCCCG2805.875336E-1012.8545734
CTCGTAT2805.8935257E-1012.852324544
GAAACGG19500.012.8024666
GATCGGA2909.622454E-1012.4606571
TCTCGTA3001.7516868E-911.99590343
GGAAACG20600.011.7154535
ATCTCGT3753.8198777E-1111.39610842
ACCGTGC6000.011.2477531
CGCCCGG5300.011.03571235
ACCGCGC4001.2369128E-1010.68536231
CCGTGCC6200.010.15925832
CCCGGAA2901.882896E-610.1242841
CGGGAAT6100.09.95453910
ACACGTC4102.1555024E-99.87360913
ATCGGAA4355.4387783E-109.8525892
Produced by FastQC (version 0.11.5)