FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6431096
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3750.016.7927844
CGTTGGA18800.014.2898981
GATCGGA4350.014.0124641
TCTCGTA4550.013.84020443
TCGTATG4800.013.61458445
ATCGGAA4900.013.3551492
AAACGGG20800.013.1913427
AACGATC5000.013.04459638
AACGGGA16950.012.7377888
GTCACGT5600.012.04914129
GAAACGG22250.011.7253896
ATCTCGT5600.011.64677942
ACGGGAT13150.011.6298919
GGAAACG22250.011.5601675
AGTCACG5950.011.34036828
ACACGTC5850.010.7645213
CGTCTGA6450.010.46053816
ACGTCTG7100.010.45317215
CGGGATT12700.010.44818310
AAACGAT7150.010.38023437
Produced by FastQC (version 0.11.5)