FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7987318
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20150.012.7574761
TATGCCG3501.0913936E-1112.2149673
AACGCGA1350.003945475411.692281
TAGTCGA1350.00400954511.66709835
AAACGGG20250.011.5546827
AACGGGT5250.010.71340758
GAAACGG21150.010.6387586
CTCGTAT4853.6379788E-1210.20528542
AACGGGA17000.010.1903418
GGAAACG23900.09.7909125
ACGGGAT12900.09.5922379
CGTATGC5201.6370905E-119.51833144
CGGGATA3351.2208111E-69.40221410
ACGGGTT5251.9645086E-108.9992629
TCGTATG5607.4578566E-118.83850643
CGGGATT12150.08.70299110
ATCTCGT5751.2914825E-108.60831440
TCTCGTA5555.857146E-108.51313541
ATGCCGT5101.0011718E-88.3824544
CCCCGAT3003.0937334E-48.2681121
Produced by FastQC (version 0.11.5)