FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6701945
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22650.012.779341
AACGGGA18750.012.1118588
AAACGGG23450.012.081437
GAAACGG22850.011.6113636
ACGGGAT13400.011.5780389
CGGGATT13450.010.86630210
CCTTCGA3805.9626473E-910.116541
GGAAACG25900.09.9455725
CGGGAAT9350.09.61920310
GCGTAAG2352.56596E-49.56846522
CGATAAA2606.335564E-59.5672251
ACAGGCG24350.09.5111318
CGCGCCC4355.977199E-99.30589533
ACCGCGC4601.5825208E-99.28903831
GCGTGAG16700.09.15593222
CCCCGGA3003.0375099E-59.0453771
CCCGAAT5751.2732926E-119.0453761
CACCGCG5505.2750693E-118.99576630
CCCCGAT2555.4929627E-48.8680161
CCCAAAG76800.08.8333761
Produced by FastQC (version 0.11.5)