FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5137805
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2500.018.8992645
CTCGTAT2600.016.44278543
TCTCGTA2850.015.78993142
ATCTCGT3300.013.63675941
CGTTGGA15550.013.3173391
ACGCCCG2857.767085E-1012.63059234
TCGTATG3501.364242E-1011.57176444
ACACGTC3753.8198777E-1111.40044112
CGCCCGG5300.011.03687435
ACGGGAT9600.011.0150869
AACGGGA13300.010.9957048
CGGGATT9200.010.76034410
ATCGGAA3805.693437E-1010.6622511
AAACGGG15650.010.6384587
GGAAACG16300.010.4928385
TCGGAAG4304.1836756E-1110.4693952
ATAGGCG2006.212922E-410.124918
GCGTGAG12250.010.10095822
ACAGGCG17700.010.042273518
CGGCCTA1800.002955173410.00067939
Produced by FastQC (version 0.11.5)