FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6825250
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18050.013.8933331
ACGGGAT11800.013.5302449
CGGGATT11350.013.07607310
AACGGGA16550.012.3643898
AAACGGG20300.011.8531277
GAAACGG20150.011.830286
GGAAACG21850.011.2667415
CCGTATC3002.7155693E-710.49364838
CCTGCGA2901.8751816E-610.1275881
CGAGGAA4458.1618055E-99.1384641
CGTGAGC17350.09.07284923
CCCGATT2000.00681742659.0369251
ACTGCGC5251.9826984E-108.99547831
AGTTCCG3301.0294123E-58.85918234
TATCTCG3155.751533E-58.56674641
TCTCGTA3451.8032268E-58.47393943
GCGTGAG17250.08.47362922
ACGCCCG3755.648528E-68.3957834
ACAGGCG23300.08.29969818
CGGCTAA6603.092282E-118.17686839
Produced by FastQC (version 0.11.5)