FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379549
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2900.014.73378344
CGCGCCC2254.942194E-913.99358533
TCTCGTA3201.8189894E-1213.3524943
ACCGCGC2959.822543E-1112.96060431
CACCGCG2959.822543E-1112.96060430
TCGTATG3500.012.88759745
CGATAGA1250.00218688112.6502691
CGCCCGG3900.012.109694535
GCGCCCG3356.366463E-1112.08401234
CCGCGCC3102.935849E-911.6079832
AAACGGG14600.011.5517367
ACGCCCG1600.001113235411.24484534
CGGGATT7600.011.2436910
AACGGGA12600.011.0652188
CCCCGTT2056.153356E-511.0193991
CGTATGC2056.25534E-511.00160845
ACGGGAT8150.010.7608319
CGTTGGA14150.010.6962151
ATCTCGT4253.45608E-1110.582942
GCCACCG8000.010.40231228
Produced by FastQC (version 0.11.5)