FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6859256
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG12400.013.2899222
CGTGAGC13600.012.55818523
CCCAACG1906.0481398E-912.287411
CGTTGGA14150.011.9892691
ACAGGCG16950.011.84220718
CCCAAAG60400.011.7761131
CACCGCG5600.011.51009430
GAGCCAC36450.011.43028426
AACGGGA12600.011.4122938
GCCACCG12400.010.99944128
AAACGGG15550.010.788477
CCCGAAT4200.010.7466081
AGGCGTG16450.010.3813920
CCCAAGT24300.010.3760351
GAAACGG16350.010.3522066
ACGGGAT9700.010.3460679
CAGGCGT16050.010.17329619
GGCGTGA16400.010.13938221
CGGGAAT5100.09.98575610
GGAAACG16250.09.955035
Produced by FastQC (version 0.11.5)