FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11890161
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA37000.012.2627331
AAACGGG38300.011.748217
CGGGATT23900.011.20156310
ACGGGAT24950.011.0908319
AACGGGA31500.011.0700778
GAAACGG39800.010.6271116
GGAAACG40800.010.1460775
GCGTGAG31200.09.73471522
ACGCCCG7100.09.50616234
CGTGAGC32950.09.49081423
ACAGGCG43250.09.46725218
CCCCGTA3104.4427143E-69.4661781
AACGGGT8900.09.3527918
ACCACGC24050.09.1672831
GCCACCG27100.09.13168828
ACCGCGC10050.08.95413131
AGGCGTG40450.08.78774120
CCCCGAT3104.5614477E-58.738011
CGGCTAA12450.08.67389539
CAGGCGT38700.08.66191619
Produced by FastQC (version 0.11.5)