FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8155569
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23500.012.8527821
AAACGGG25400.012.4866917
AACGGGA22050.012.3435128
GAAACGG26050.012.0041196
ACGGGAT16150.011.6995389
CGGGATT15400.011.39294110
GGAAACG26900.010.9753365
CCCGAAA3806.2882464E-910.0838311
TCTCGTA3309.973392E-79.54280641
CTCGTAT3552.6352427E-79.50446142
ACCGCGC6050.09.29494131
CACCGCG6650.09.13280330
GCGTGAG20500.08.99788922
CCCGGAT3552.61475E-68.8891421
CGTGAGC21900.08.83354423
CCCGTTA2350.0026651068.6324641
CGCCCGG10450.08.61078935
ACGCCCG5607.003109E-108.43588334
CGGGAAT10400.08.43515810
ACAGGCG29850.08.36451218
Produced by FastQC (version 0.11.5)