FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11012995
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1765361.6029790261413903TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA191250.043.8641741
TCGTATG190050.043.5856945
TCGGAAG194050.043.264953
ATCGGAA194150.043.233032
TCTCGTA191500.043.165843
TATCTCG191900.043.1467441
CTCGTAT191900.043.12270744
ACACGTC195150.043.096913
CGTCTGA196200.042.85518616
ATCTCGT193200.042.8441942
ACGTCTG198000.042.48715215
CGGAAGA197650.042.4651374
CACGTCT199000.042.30832714
GCACACG201550.041.78420611
CACACGT204200.041.2199212
TCTGAAC218500.038.60494218
AGAGCAC219750.038.4261138
AGCACAC220300.038.31979410
GAGCACA220600.038.2678539
GTCACAT219600.037.9603529
Produced by FastQC (version 0.11.5)