FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5312750
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16600.014.6902411
AACGGGA14050.013.7696218
AAACGGG17200.013.7328527
ACGGGAT10050.013.6541189
GGAAACG18600.013.5458095
CGGGATT9600.013.35733510
GAAACGG18950.013.0582016
AACGGGT3356.366463E-1112.0872518
CCCCGAT1600.00107681911.2897221
AACGGTC1650.001436444210.9070158
CGGGAAT6100.010.69508810
GCGCCCG3602.6575435E-910.62547834
CGCACCC2657.4095788E-610.18932933
CCCCGAA2006.014219E-410.160751
ACCGCGC2901.9796917E-610.08466731
CACCGCG3351.2189994E-69.40305430
CGTGAGC12450.09.39633323
ACGGGTT3603.2234675E-79.3732169
CCTCGTT2200.00144012399.2370451
CTCGTAT2200.00148796349.20400641
Produced by FastQC (version 0.11.5)