FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9167923
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG2550.016.76362431
ACGGGAT15350.012.8989639
CGTCCGC3405.456968E-1212.57271832
CGTTGGA26950.012.29359151
AACGGGA21100.012.2629778
AAACGGG28000.012.0527277
CGGGATT13450.012.04455510
GAAACGG27850.011.9560756
GTCCGCA3804.7293724E-1111.24951833
CGTATGC5000.011.24884445
GGAAACG28800.011.1721295
TCTCGTA4101.6370905E-1110.97478142
TCCGCAC4152.419256E-1010.30076434
CTCGTAT4702.1827873E-1110.05243243
AACGGGT7200.09.3749578
CGATAAG2000.00695321769.0153011
ACGCCCG5007.403287E-108.99961634
ACAGGCG32350.08.97130618
CGGGATA4051.8471837E-78.88884810
GCCACCG19450.08.79120628
Produced by FastQC (version 0.11.5)