FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8489485
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2650.013.5949471
CGCTAGT900.00398861111.6752911
GCGTGAG13000.011.65311922
ACGGGAT13750.011.3446799
CGTTGGA19250.011.3070541
CGGGATT11450.011.13454510
ACAGGCG17600.010.99350318
AAACGGG20950.010.8106537
AACGGGA17400.010.7751268
ATCGGAA3950.010.63575652
CGTGAGC14200.010.45709423
CGTCTGA3650.010.27321116
ACACGTC3550.010.14009313
CCCAAAG65100.010.0304511
GAAACGG21600.09.9992586
CCCGAGT12700.09.9285941
CCGAGTA9200.09.7852252
GAGCCAC39150.09.693109526
TACAGGC45750.09.605844517
TCGGAAG4100.09.5138993
Produced by FastQC (version 0.11.5)