FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9322119
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23700.011.88896751
AACGGGA22100.011.8066618
AAACGGG26850.011.561027
GAAACGG28500.011.1303846
GGAAACG28600.010.7177595
ACGGGAT15800.010.5350359
CGGGATT15350.09.96464610
GGTAACG2200.00146261399.2215162
CCCCGAT3104.6369776E-58.7257351
CGGGAAT11100.08.71378510
CGGCTAA9300.08.46583439
ACGGGAA10800.08.3310099
ACCGCAC8600.07.846647731
CCGGCTA9200.07.824323738
CGTCTAA3501.9072232E-47.72850851
GCCACCG19800.07.49790828
ACCCGGC8553.6379788E-127.366760736
ACGCCCG5251.1914472E-67.28407334
CCCTAAC16000.07.18509671
GAGGTCG6601.6412741E-87.17194274
Produced by FastQC (version 0.11.5)