FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6449641
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16350.015.1876611
ACGGGAT11700.013.6470569
AACGGGA16650.012.9665218
AAACGGG19250.012.7339247
CGGGATT11650.012.16132910
GAAACGG21150.011.5925846
GGAAACG21850.011.1527565
CCCGTAT2006.0270017E-410.1584741
CGTTGGT3053.765316E-58.8817261
CGGGAAT7950.08.48634310
ACAGGCG24350.08.40444318
CGTGAAA5708.109055E-97.92083841
GCGTGAG16500.07.90515722
GTTGGAA35000.07.86505272
CCCCGGA4903.7650716E-77.831931
CCGAATT2600.00656625867.8105032
CGTGAGC16650.07.563803723
CAGGCGT21850.07.410519
CCCAAAG69700.07.31967261
GATTTCG8054.0017767E-117.2876475
Produced by FastQC (version 0.11.5)