FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7291662
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT14100.014.6715649
AACGGGA19050.014.4003338
AAACGGG21450.014.0468617
CGTTGGA21050.013.63758851
CGGGATT13100.013.216825510
GAAACGG23400.012.7801976
GGAAACG24450.012.6579775
CCCCGAT2351.6642807E-611.5424851
CGCAATC3602.2555469E-1011.24303821
GCGCAAT3401.2501914E-810.58146520
CGGGAAT8400.09.63675810
GGCGCAA3502.1556843E-79.63669119
CCCGGAT2453.6981373E-49.2261361
ACGCCCG3901.0308722E-68.64837334
CGTGAGC17450.08.50499623
ACCGCGC5058.463758E-98.46155831
CCCGGAA3806.3009484E-68.3278021
GCCACCG14350.08.30638628
GCGTGAG17800.08.21126422
ACAGGCG24750.08.17664218
Produced by FastQC (version 0.11.5)