FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10963454
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACA154450.14087713598287546TruSeq Adapter, Index 5 (100% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG123720.11284764819554129TruSeq Adapter, Index 5 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19900.026.036361
ATCGGAA35450.014.595932
TCGGAAG36000.014.4562593
ACACGTC35200.014.23071813
CGTCTGA35650.014.09386316
CGGAAGA37450.013.8963464
ACGTCTG36850.013.59426515
CACGTCT37600.013.36299214
GCACACG37950.013.35788811
CACACGT39050.013.09683212
CGTTGGA24850.012.0008961
ACGGGAT16300.011.4094579
GTCACAC48500.011.10129629
AACGGGA20950.011.0962598
CGTGAGC17950.010.86180123
GCGTGAG18650.010.45407322
AGAGCAC50700.010.4422888
AGCACAC51800.010.33644910
GAGCACA52300.010.2375829
AAACGGG27050.010.2018627
Produced by FastQC (version 0.11.5)