FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4933763
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2500.022.50088742
TCTCGTA2750.020.4553541
GATCGGA3150.018.616321
CGTATGC3350.018.1354144
TCGTATG3650.016.0280343
TCACGAT3550.015.8445735
ATCTCGT3750.015.60061540
CGTTGGA16250.014.1572151
ACGGGAT10100.013.8093479
ATCGGAA4250.013.7667852
ACACGTC4250.013.764132513
CACCGCG3500.013.500669530
AACGGGA14500.013.4975228
AAACGGG17650.013.1297657
GAAACGG17500.013.1154676
GCCACCG8450.012.78027328
GGAAACG18150.012.3978115
ACCGCGC3457.2759576E-1212.39204331
AACGGGT2552.8416252E-812.3506758
CGGGATT9700.012.29330310
Produced by FastQC (version 0.11.5)