FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10425717
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA5350.013.88038129
TCTCGTA6000.013.12620741
CTCGTAT5750.012.91305442
GATCGGA5800.012.4210131
CGTTGGA33500.011.5589571
CGTATGC6300.011.42907444
ATCTCGT6700.011.08348140
ACGGGAT21600.011.0405119
AACGGGA28800.011.0144738
CGATCAG6400.010.90032933
ACCGCGC7750.010.74379931
AAACGGG35700.010.4603887
ATCGGAA7750.010.4535772
TCGTATG6900.010.43479243
CACCGCG8500.010.32517230
AGTCACG7250.010.24251828
CGGGATT19900.010.17481410
GAAACGG38150.09.7296516
GCGTGAG26450.09.61133222
GGAAACG38400.09.4904655
Produced by FastQC (version 0.11.5)