FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428778
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3350.020.18770445
TCTCGTA3550.016.47092842
CTCGTAT3700.015.80318943
TATCTCG3900.014.99290740
CGTAACC950.005267410514.2556754
CGTTGGA17350.013.9475041
GTCACGA4500.013.49510828
TCGGAAG4900.013.3586362
ATCTCGT4700.012.919341
AACGGGT2857.8216544E-1012.6239788
ATCGGAA4850.012.5902461
GAAACGG19250.012.5033446
AAACGGG19650.011.9012327
GGAAACG19650.011.8313665
CGGGATT11600.011.82477810
AACGGGA16750.011.8137838
TCGTATG5150.011.78934444
ACGGGAT12600.011.7786239
GATCGGA3002.0689185E-811.3079071
ACACGTC5450.011.14008212
Produced by FastQC (version 0.11.5)