FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11891590
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5400.017.08319945
CTCGTAT6500.013.84627741
CGGGATT17850.013.61299310
ACGGGAT19550.013.349949
CGTTGGA31850.013.148681
AACGGGA27800.012.7064138
TCTCGTA7100.012.67611640
ACCGCGC8000.012.37401731
AAACGGG34000.012.242247
GAAACGG34100.012.1403616
CACCGCG8500.011.911118530
CGTATGC7850.011.75135143
GGAAACG36800.011.008825
CCGCGCC9050.010.93835832
ATCTCGT9300.010.88714739
TCGTATG8850.010.67774742
CGCGCCC7100.010.45691733
ACAGGCG37550.09.94644918
GCGTGAG28600.09.83330122
GCCACCG25050.09.52044328
Produced by FastQC (version 0.11.5)