FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305797
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25600.014.7943321
AAACGGG24800.014.5151967
ACGGGAT15500.014.2256629
AACGGGA21850.014.1074088
GAAACGG26250.013.9709516
CGGGATT14800.013.53026710
GGAAACG27050.013.3082315
CCCGTAA4253.601599E-1010.078381
CGCCCGG9250.09.97227135
GCGTGAG21150.09.57379422
ACCGCGC6400.09.49163331
ACAGGCG29300.09.44506918
GCCACCG18550.09.21779128
GCCCGGC19900.08.93153236
CGTGAGC21700.08.91642323
TCTCGAC3053.9173603E-58.85194231
CGGGAAT11000.08.79536510
TGCCCGG11300.08.76044635
ACGCCCG4651.8586434E-88.709064534
CCCAAAG92600.08.6669251
Produced by FastQC (version 0.11.5)