FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5002355
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5150.022.71228644
TCTCGTA5200.021.19617341
CTCGTAT5350.020.60188942
TCGTATG5750.020.34230643
GATCGGA6150.019.085811
ATCTCGT6200.018.1402440
ATCGGAA7800.015.6002982
CATCTCG7300.015.40677839
ACACGTC7900.015.09084213
CGTCTGA8200.014.53873816
TCGGAAG8450.014.4002753
TATGCCG2102.9564035E-813.94291245
GTATGCC8700.013.20323545
CGTTGGA13200.012.9963681
CACCGCG3202.910383E-1112.65281830
ACGTCTG9350.012.50997515
CACGTCT9650.012.35416114
CGGAAGA10150.012.2104134
ACCGCGC3203.783498E-1011.94988331
GCACACG10650.011.82777811
Produced by FastQC (version 0.11.5)