FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11628891
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6000.014.8969261
ATCGGAA7950.012.4490822
CGTCTGA7700.012.07335216
ACACGTC7600.012.03491913
CGTTGGA30300.011.8495441
ACGTCTG7800.011.7263315
TCGGAAG8400.010.8896253
CACGTCT8950.010.55466514
CGGGATT16750.010.47360210
GCGTGAG18650.010.45275222
AAACGGG31150.010.3973487
AACGGGT6250.010.3160958
ACGGGAT18900.010.3135569
GAAACGG32100.010.0899436
CGTGAGC18600.010.07774123
GGAAACG31950.010.0439685
ACAGGCG25500.09.87861318
CGGAAGA9600.09.840834
GCCACCG16700.09.69713728
CCCAAAG88250.09.6818931
Produced by FastQC (version 0.11.5)