FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7164670
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA14950.012.3401298
CGTTGGA19550.012.1507741
AAACGGG19750.011.4674187
GCGTGAG13300.011.16450222
ACAGGCG18100.011.10406618
ACGGGAT11350.010.836129
GAAACGG19950.010.8261836
GGAAACG19700.010.5828915
CGGGATT11550.010.51862110
CGTGAGC13600.010.47715923
CACCGCG4600.010.43407630
ATAGGCG2752.1645974E-1010.3627918
GCCACCG11300.010.08774228
CCCAAAG59950.09.7054421
CCCAAGT25500.09.6693491
AGCGGCT4500.09.6658774
CCCTAAA16800.09.6651381
CCACCGC8850.09.660295529
AGGCGTG17650.09.60261520
GGCGTGA16550.09.33458521
Produced by FastQC (version 0.11.5)