FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12393256
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCG8250.011.81875130
CGTTGGA26950.011.0220511
AACGGGA23850.010.5653948
ACGGGAT18100.010.2756189
GCCACCG20300.09.90211528
GAAACGG29150.09.8793316
GCGTGAG23450.09.851322
ACAGGCG29100.09.84476218
AAACGGG29250.09.7942777
CGTGAGC24050.09.79268923
CGGGATT16550.09.78792510
GGAAACG30750.09.46295455
CCCAAAG98050.09.3792661
CCCAACG3306.0936145E-109.0922641
GAGCCAC64300.09.05164826
CCACCGC16500.08.90959329
AGCCACC50350.08.60997327
CGTTAAT3152.4323526E-88.5727061
CCCGAGT20350.08.5516431
CCCAAGT40200.08.5460491
Produced by FastQC (version 0.11.5)