FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7813501
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8200.032.1024945
CGTCCCG9400.028.2431134
CCCGATC10350.025.65074737
GTCCCGA10400.025.31109435
TCCCGAT11000.024.33955436
CTCGTAT10950.024.24569143
TCTCGTA10800.024.16578742
CCGATCT11300.023.29516438
ACCCGTC11400.023.0908231
TCGTATG11700.023.07608244
CCCGTCC12100.022.31280732
GATCGGA5250.022.3068871
CACCCGT11850.022.21395730
ATCTCGT11750.022.02005441
CCGTCCC12400.021.95442633
ATCGGAA12250.021.5102121
TCACCCG12450.021.5048329
ACACGTC12600.021.24963612
CGTCTGA12850.020.83581515
TCGGAAG13250.020.0567722
Produced by FastQC (version 0.11.5)