FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636254
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4650.024.67982345
TCTCGTA4700.024.4161940
CTCGTAT5050.023.16954641
CGTATGC5550.021.48743643
ACACGTC5950.020.79739412
TCGTATG6250.019.08084542
ATCGGAA6300.018.9721
TCGGAAG6500.018.348432
ATCTCGT6650.017.59382439
CGTCTGA7850.016.04967115
CACGTCT7900.015.94865613
ACGTCTG8050.015.65133714
CGTTGGA14600.014.6740011
ACCGCGC3400.013.89828931
CGGAAGA8800.013.2962783
GCACACG9550.013.1931310
CACACGT9950.013.11499411
GTATGCC9300.013.06578844
AATCTCG8750.012.85520138
CACCGCG3850.012.27370530
Produced by FastQC (version 0.11.5)