FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4553477
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG61920.13598399640538428TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8400.038.62216645
CTCGTAT9400.035.89937641
TCTCGTA9700.034.32523340
ATCGGAA10150.033.5265241
ACGTCTG10450.033.3686714
ATCTCGT10200.033.304339
TCGGAAG10250.033.164052
CGTCTGA10550.033.05237615
CGTATGC10200.032.64262443
ACACGTC10700.032.5890312
CATCTCG10600.031.83529538
TCGTATG10600.031.6230642
CACGTCT11400.030.39060413
CGGAAGA12000.028.3276273
GCACACG12350.028.2350310
GTATGCC12650.026.67680244
CACACGT13300.026.0490911
CTTAGGC15700.022.06706632
GCATCTC16800.020.75610737
ACTTAGG17750.019.7719631
Produced by FastQC (version 0.11.5)