FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6536177
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19500.012.978181
ACGGGAT13800.011.5744899
AAACGGG20500.011.3033187
CCCGGAT2004.772805E-511.297971
CGGGATT12000.011.24879110
GAAACGG20700.011.1941076
CCCGCAT2452.7580609E-611.0673991
GCGTGAG15050.011.06176522
CGCCCGG6150.010.97434735
CGGCCTA2056.416326E-510.97434639
AACGGGA17850.010.9648728
CCCCGAT2107.8405625E-510.7599711
GGAAACG21550.010.5437875
CGTGAGC16750.010.47632623
CGTATAA7150.010.1128671
GTCGTTT2150.00120666319.4178092
ACTGCGC5505.456968E-129.4091631
ACAGGCG21300.09.40032718
ATCGCGC2658.253788E-59.33854737
GCCACCG13300.09.13540528
Produced by FastQC (version 0.11.5)