FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5958089
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23550.015.079551
GAAACGG25150.012.966426
CGGGATT13900.012.77985310
AACGGGA20400.012.5657448
GGAAACG25200.012.4584435
ACGGGAT14300.012.4223759
AAACGGG25000.012.2324217
CCCGTAT1400.00505720311.3096621
CGGGAAT9500.010.41460110
AAGCGGC5550.010.1741123
CGCGCCC4702.1827873E-1110.04730433
GTTGGAA39500.010.006692
GATTTCG9400.09.8515715
AACGGGT5251.8189894E-129.8510398
GCCACCG16150.09.60729328
CGTGAGC14750.09.4519323
CCCAAAG69850.09.32622051
CACCGCG6050.09.2919230
ACCGCGC6300.09.28012131
CCCGAAA2200.0014171799.253361
Produced by FastQC (version 0.11.5)