FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4497064
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA13100.013.2628231
ACGGGAT9000.013.2439749
AACGGGA11350.012.87960158
GCGTGAG9750.012.68738122
CGCGCCC2502.159868E-812.5958133
CCCGGCC7600.012.42896837
CGCCCGG4200.012.3173335
ACCCGGC3152.910383E-1012.13881936
CGTGAGC10800.012.07864323
CGCACCC2251.0454078E-611.99600933
ACCGCGC2851.0504664E-811.83816731
AAACGGG14150.011.4435567
CCGCGCC3153.768946E-911.42477132
ACAGGCG15800.011.38722318
CCCGTAA2201.0282696E-511.2820121
CACCGCG3204.82396E-911.24625930
CGGGATT8650.011.17988410
GAAACGG15200.010.9524976
GGAAACG15900.010.5009115
AGGCGTA1500.00881342610.49522420
Produced by FastQC (version 0.11.5)