FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084031
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16800.014.5033821
AACGGGA14000.013.9769148
AAACGGG17150.013.9015057
GAAACGG17650.013.3814486
CGGGAAT7850.012.89263210
GGAAACG19050.012.6704545
CGGGATT8950.012.31322410
ACGGGAA7250.011.4784659
ACGGGAT10200.011.2457939
CCCCGGT2201.2760572E-410.2549161
AAGCGGC4051.6498234E-910.0275243
AACGGGT3603.025343E-89.9962618
AAGCGGG3858.533425E-89.3471527
GCCACCG10850.09.12083228
GTTGGAA31000.08.951552
CGTGAGC13000.08.82370923
GCGTGAG13050.08.78990222
ACCGCGC4102.2408676E-78.77703231
ACCGTGC5403.43789E-108.74655631
CGCCCGG5151.3005774E-98.73433935
Produced by FastQC (version 0.11.5)